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Abstract Microrobots hold immense potential in biomedical applications, including drug delivery, disease diagnostics, and minimally invasive surgeries. However, two key challenges hinder their clinical translation: achieving scalable and precision fabrication, and enabling non‐invasive imaging and tracking within deep biological tissues. Magnetic particle imaging (MPI), a cutting‐edge imaging modality, addresses these challenges by detecting the magnetization of nanoparticles and visualizing superparamagnetic nanoparticles (SPIONs) with sub‐millimeter resolution, free from interference by biological tissues. This capability makes MPI an ideal tool for tracking magnetic microrobots in deep tissue environments. In this study, “TriMag” microrobots are introduced: 3D‐printed microrobots with three integrated magnetic functionalities—magnetic actuation, magnetic particle imaging, and magnetic hyperthermia. The TriMag microrobots are fabricated using an innovative method that combines two‐photon lithography for 3D printing biocompatible hydrogel structures with in situ chemical reactions to embed the hydrogel scaffold with Fe3O4nanoparticles for good MPI contrast and CoFe2O4nanoparticles for efficient magnetothermal heating. This approach enables scalable, precise fabrication of helical magnetic hydrogel microrobots. The resulting TriMag microrobots, with the synergistic effects of Fe3O4and CoFe2O4nanoparticles, demonstrate efficient magnetic actuation for controlled movement, precise imaging via MPI for imaging and tracking in biological fluid and organs, including porcine eye and mouse stomach, and magnetothermal heating for tumor ablation in a mouse model. By combining these capabilities, the fabrication and imaging approach provides a robust platform for non‐invasive monitoring and manipulation of microrobots for transformative applications in medical treatment and biological research.more » « less
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Wang, Jiaqi; Xing, Yi; Ngatio, Michael; Bies, Paulina; Xu, Lu_Lucy; Xing, Liuxi; Zarea, Ahmed; Makela, Ashley_V; Contag, Christopher_H; Li, Jinxing (, Advanced Materials)Abstract Nature's ability to create complex and functionalized organisms has long inspired engineers and scientists to develop increasingly advanced machines. Magnetotactic bacteria (MTB), a group of Gram‐negative prokaryotes that biomineralize iron and thrive in aquatic environments, have garnered significant attention from the bioengineering community. These bacteria possess chains of magnetic nanocrystals known as magnetosomes, which allow them to align with Earth's geomagnetic field and navigate through aquatic environments via magnetotaxis, enabling localization to areas rich in nutrients and optimal oxygen concentration. Their built‐in magnetic components, along with their intrinsic and/or modified biological functions, make them one of the most promising platforms for future medical microrobots. Leveraging an externally applied magnetic field, the motion of MTBs can be precisely controlled, rendering them suitable for use as a new type of biohybrid microrobotics with great promise in medicine for bioimaging, drug delivery, cancer therapy, antimicrobial treatment, and detoxification. This mini‐review provides an up‐to‐date overview of recent advancements in MTB microrobots, delineates the interaction between MTB microrobots and magnetic fields, elucidates propulsion mechanisms and motion control, and reports state‐of‐the‐art strategies for modifying and functionalizing MTB for medical applications.more » « less
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